How to create 3D from 2D slices?

You can load a stack of images as described here, then remove the Matlab plot axes by using Crop volume.

You can then go to Data module and drag-and-drop the image to a 3D view to see it with volume rendering:

I would recommend to save the image array as nrrd (for example, using nrrdwrite.m) instead of saving resampled, color-mapped screenshots.