# How to handle .tif file from MIB

**URL:** <https://discourse.slicer.org/t/how-to-handle-tif-file-from-mib/12318>\
**Category:** Support\
**Tags:** segmentation\
**Created:** [July 1, 2020, 1:11pm UTC](https://discourse.slicer.org/t/how-to-handle-tif-file-from-mib/12318 "2020-07-01T13:11:50Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![Linford\_Briant](https://sea2.discourse-cdn.com/flex002/user_avatar/discourse.slicer.org/linford_briant/32/7309_2.png) [@Linford\_Briant](https://discourse.slicer.org/u/Linford_Briant)\
**Post date:** [July 1, 2020, 1:11pm UTC](https://discourse.slicer.org/t/how-to-handle-tif-file-from-mib/12318/1 "2020-07-01T13:11:50Z")

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Dear Slicer Forum,

I am new to Slicer, and using it to view in 3D images I have segmented in MIB. (These are serial blockface EM images.) I have saved the images from MIB as a .tif file and I import it into slicer, and then use the volumes model to view the segmentations in 3D.

However what I can’t figure out is:

1. How do I assign different colours to my different segments? (See axial view in the image - I want these 4 objects to be different colours both in 2D and the 3D module.)

2. How do I smooth these objects in 3D?

3. How do I connect the 2D slices together in 3D, as they appear to be rendered as a series of flat disks rather than a 3D surface.

Any help would be really appreciated!

 ![screenshot3](https://us1.discourse-cdn.com/flex002/uploads/slicer/original/3X/b/d/bd93e9ddc402b85dbd14cbaddf0840e4a5de0757.png)

Kind regards,  
Linford