# Segmenting vasculature from two-photon imaging

**URL:** <https://discourse.slicer.org/t/segmenting-vasculature-from-two-photon-imaging/8476>\
**Category:** Support\
**Created:** [September 18, 2019, 1:29am UTC](https://discourse.slicer.org/t/segmenting-vasculature-from-two-photon-imaging/8476 "2019-09-18T01:29:40Z")\
**Posts on this page:** 1\
**Showing post:** 15

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**Author:** ![pieper](https://sea2.discourse-cdn.com/flex002/user_avatar/discourse.slicer.org/pieper/32/8_2.png) [@pieper](https://discourse.slicer.org/u/pieper)\
**Post date:** [August 29, 2023, 12:43pm UTC](https://discourse.slicer.org/t/segmenting-vasculature-from-two-photon-imaging/8476/15 "2023-08-29T12:43:59Z")

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> [@ylcnkzy](#):
>
> bigger data such (probably at 1-5 GB in size), do you think if 3DSlicer able to render such big data?

That should be no problem if you have enough memory, although some steps, like decompressing, are single threaded and can be slow. Cropping and downsampling can help a lot when exploring the data.

This example data was something like 180GB but can be explored at full res (not volume rendered).

> [@Imaging Data Commons July 2023 release](https://discourse.slicer.org/t/imaging-data-commons-july-2023-release/30648/7):
>
> As far as I know the VHP Female data digitized from the 70mm film photos is the highest quality available. It is very interactive to view if you have a big enough machine: [[VHP F 70mm film] ](https://www.youtube.com/watch?v=ltNLT4jUX58) It would be great to hear if people know of higher quality data. Or non-human data like this of high quality could also be interesting to look at.

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