# Tractography issue

**URL:** <https://discourse.slicer.org/t/tractography-issue/24093>\
**Category:** Support\
**Tags:** segmentation, diffusion, tractography, dti\
**Created:** [June 29, 2022, 4:40am UTC](https://discourse.slicer.org/t/tractography-issue/24093 "2022-06-29T04:40:03Z")\
**Posts on this page:** 5\
**Page:** 1

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**Author:** ![massimo](https://avatars.discourse-cdn.com/v4/letter/m/7ba0ec/32.png) [@massimo](https://discourse.slicer.org/u/massimo)\
**Post date:** [June 29, 2022, 4:40am UTC](https://discourse.slicer.org/t/tractography-issue/24093/1 "2022-06-29T04:40:03Z")

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Operating system: Mac OSX Monterey  
Slicer version:5.0.2  
Expected behavior: Tractography visualization  
Actual behavior: Errors in data import

Dear Community,

I’m trying to create a 3d model of a tractography to view the that i just received.

I followed the various tutorial provided by this community but as soon as i try to import the DICOM’s set, i notice a serie of errors.

The DICOM files that i received are without extension .dcm, .nii, nifti, nrrd

I installed the UKFTractography, SlicerDMRI and SlicerDcm2nii

Following the link of the DICOM’s set.

I think I’m doing something wrong so I hope someone with more experience could indicate me the right process to follow.

Best regards

Massimo

https://…

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**Author:** ![massimo](https://avatars.discourse-cdn.com/v4/letter/m/7ba0ec/32.png) [@massimo](https://discourse.slicer.org/u/massimo)\
**Post date:** [June 29, 2022, 6:55am UTC](https://discourse.slicer.org/t/tractography-issue/24093/2 "2022-06-29T06:55:02Z")

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Following the errors track

Could not load: Design - as DWI Volume as a Diffusion Volume (please see DWIConvert module for advanced options and help)

Could not load: ep2d\_diff\_mddw\_20\_p2\_TRACEW - as DWI Volume as a Diffusion Volume (please see DWIConvert module for advanced options and help)

Could not load: 15: ep2d\_diff\_mddw\_20\_p2\_TENSOR as a Scalar Volume

Could not load: ep2d\_diff\_mddw\_20\_p2\_ColFA - as a 40 frames MultiVolume by ImagePositionPatient+AcquisitionTime as a MultiVolume

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**Author:** ![pieper](https://sea2.discourse-cdn.com/flex002/user_avatar/discourse.slicer.org/pieper/32/8_2.png) [@pieper](https://discourse.slicer.org/u/pieper)\
**Post date:** [June 30, 2022, 2:17pm UTC](https://discourse.slicer.org/t/tractography-issue/24093/3 "2022-06-30T14:17:27Z")

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@massimo you will need to install SlicerDMRI and make sure the dicom diffusion plugin is enabled.  
From there you can load the `ep2d_diff_mddw_20_p2` series and load it as DWI. From there you can do interactive and whole brain tractography and other SlicerDMRI operations. Most of the other diff series are pre-calculated from the DWI on the scanner.

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**Author:** ![massimo](https://avatars.discourse-cdn.com/v4/letter/m/7ba0ec/32.png) [@massimo](https://discourse.slicer.org/u/massimo)\
**Post date:** [June 30, 2022, 5:57pm UTC](https://discourse.slicer.org/t/tractography-issue/24093/4 "2022-06-30T17:57:56Z")

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Thanks for your kind reply,  
I already installed SlicerDMRI

 ![image](https://us1.discourse-cdn.com/flex002/uploads/slicer/original/3X/a/3/a3b09b298ae66761b84ce19d5afe24c273fca7c7.jpeg)

To be more accurate, i do the following:  
Menu/Diffusion/Import And Export/ Tractography DICOM load

Unfortunately, during the import, I notice these serie of errors:  
Could not load: Design - as DWI Volume as a Diffusion Volume (please see DWIConvert module for advanced options and help)  
Could not load: ep2d\_diff\_mddw\_20\_p2\_TRACEW - as DWI Volume as a Diffusion Volume (please see DWIConvert module for advanced options and help)  
Could not load: 15: ep2d\_diff\_mddw\_20\_p2\_TENSOR as a Scalar Volume  
Could not load: ep2d\_diff\_mddw\_20\_p2\_ColFA - as a 40 frames MultiVolume by ImagePositionPatient+AcquisitionTime as a MultiVolume

So cannot proceed.  
Did you try to import the same serie? In case, any errors?  
Thanks again  
Massimo

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<div class="post-metadata">

**Author:** ![pieper](https://sea2.discourse-cdn.com/flex002/user_avatar/discourse.slicer.org/pieper/32/8_2.png) [@pieper](https://discourse.slicer.org/u/pieper)\
**Post date:** [June 30, 2022, 6:21pm UTC](https://discourse.slicer.org/t/tractography-issue/24093/5 "2022-06-30T18:21:55Z")

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I was able to load and do tractography via the DICOM module from the `ep2d_diff_mddw_20_p2` series, so focus on that one and you should be able to load it too. The other series are derived data that cannot be used for tractography.

Specifically:

- import the study into the DICOM database
- select only the `ep2d_diff_mddw_20_p2`
- in the DICOM Plugins tab be sure DICOMDiffusionVolumePlugin is select and turn off the others
- clicking Load should load data as a diffusion volume
- you will see the baseline volume, but if you go to the Volumes module you can scroll through the diffusion directions
- you can then use any of the SlicerDMRI operations (tensor estmation, interactive tractography, UKF, etc)