3D reconstruction from 2D slices

Many thanks for your response. I too have a 4GB GPU on my laptop. Nvidia GeForce 940MX.

I switched to VTK CPU Ray Casting In the Volume rendering tab( I think this is selected by default). Physical memory is 8GB)

I navigated to “Volumes” option and I have the following settings. Active volume is selected automatically. For now, I am using the default Image Spacing. I will write to the authors and find out the actual spacing. In the paper it is mentioned, “To prepare the image stack for
importation to Imaris, the image stack file was converted to 8-bit, noise was filtered out using a (3×3×3) median filter, and the pipette image and other artefacts were subtracted out”


Could you please suggest how to proceed from here?