I am looking for a way to extract data from stacked fluorescent image data of specific tip-growing fungi. I already remodeled the surface and reduced it to a centerline (which i managed to load into 3Dslicer as a Surface file). Now I would like to stepwise reduce dimensionality by walking along the centerline, averaging the intensities over circle planes perpendicular to it upto the apex.
Do you have any tool or workflow in mind to do this?
Thx in advance, Philipp