Landmark registration malfunction

Hello,

I am using the Landmark Registration module to perform registration of a post mortem ex vivo hemisphere on MRI on a normal brain MRI atlas to correct for the distortion that occurs due to absence of the skull in post mortem ex vivo scanning. As I am using brains with Alzheimer’s disease, the brains show a lot of atrophy and a lot of distortion.

After performing the landmark registration, it looks like this:

To save the transformed hemisphere, I need to use the Harden transform button. However, when I click it the transformation changes and the registration becomes totally inaccurate.

Afbeelding2

Could you explain what the Harden transform button does exactly? Any ideas how to avoid this change of transformation?