Error of new releases of 3D slicer

I would suggest you check your masking options as it sounds like your segment 2 is overwriting your segment 1. Please read Segment editor — 3D Slicer documentation.

Your steps leading up to when it is not working for you would be helpful along with any screenshots. What type of volume is it? I can only think that you may be running into a limitation of using multi-volume rendering with the maximum intensity projection option instead of composite shading. See related post below:

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