How to set dimensions and slice thickness when reconstructing FIB stack

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1

Hi,

I’m using 3D Slicer to reconstruct a material volume. The slices of this volume were createt using FIB (Focused Ion Beam) in a SEM. At this point I struggle to set the correct dimensions of my volume. Each image of my stack is 250x250 pixel and 1 pixel is equivalent to 0.025µm. My stack consists of 199 images, where one image reprents one slice created using FIB with a slice thickness of 40nm. How can I implement the slice thickness of 40nm and the correct dimensions to have a correct length bar?

2

After you import your data, you can edit those fields in the Volumes module. From your description it sounds like spacing should be (0.000025, 0.000025, 0.00004). Default units in slicer is millimeters.

3

Thank you very much!
I changed the values and verified them by using the measurement tool and indeed it shows the correct distance.

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