I am using the “Remove Small Islands” function with a voxel size of 10 after segmenting with the threshold function.
The information of the volume I’m working with is the following:
No, that is not correct. Based on the information above, your voxel size is anisotropic. it is 0.00045x0.00045x0.005 (in other words about 0.45 micron in XY and 5 micron in Z axes).
How this applies to the voxel size for “Remove Small Islands” from a segment?
Does a voxel size of 10 correspond to dimensions of 4.5 × 4.5 × 50 microns?
When I apply it to my segments, this doesn’t seem to be the case. Graphically, it appears to remove only very small islands, around 4.5 microns³.
Do segments always have isotropic voxels?
This is very hard to confirm visually. If you have any doubts, paint a blob of 10 voxels then one of 9 and see if it’s correct. By the way this part of the code has not changed for about 7 years. Let us know if you do find an issue. (I just tried it and it works as expected)
No. A segmentation assumes the geometry of its source volume unless you change it manually.