Strategies for working with large microCT files

Hi,

I am working with microCT tiff stacks and currently it is 75GB when read into the software at full resolution. Following the general guideline of using a hardware with RAM 6x-10x that of the file is not attainable especially with the current global memory shortage. I have been fortunate enough to get my hands on an HPC with 800GB of RAM and thus am able to do basic segmentation using the thresholding tool. However, it still takes a quite a while to convert the segments into 3D so that I can verify the segmentation.

Things I’ve tried:

Questions:

  • If I don’t use any semi-automated segmenting tools, could I subset the object using ROI into sections to do my segmentation and then combine the segmentation from each section together afterwards? This way I will only have to work with 1/4 of the data at a time.
  • What other strategies or recommendations are there for segmenting with very large files?

Thanks,