Im working on a project where i need to determine the volume of fibrosis in cochlea’s. the raw data consists of +/- 100 microscopic photo’s of the cut up cochlea that i aligned and stacked in order(.tiff file). I coloured the segments ( bone; internal space; fibrosis) and tried to use segment statistics to find the volumes of the different segments. when i did this however i got volumes in the thousands cm3. The size differences between the segments seem right but the scale is not.
Can i fix this in a way that will get me an accurate volume size?