Hi,
I am a beginner at 3D Slicers and would like some suggestion on how can I subtract volumes from two different CT scans. My goal is obtained the amount (Volume) of bone removed from the first image by comparing to it the second volume.
To create subtraction images you can use Subtract Scalar Volume module that is pre-built inside 3D Slicer. Alternatively, you can use Python and perform simple subtraction between two volumes. Relevant thread: Scalar subtraction
However, if you are calculating difference between two segmentations (bone segmented on image 1 - bone segmented on image 2), you don’t need to subtract them, just segment both and calculate their respective volumes with Segment Statistics module.
So to answer your original question – how to subtract volumes from two different CT scans – look at my post above: you can use either Subtract Scalar Volume or python.