Transform and Harden to establish Orthogonal view

Support
1

Hello Everyone, I have a problem with my scanned fish heads (from µCT, tiff-files).

My scans are shifted and I wand to bring them in an standardized orthogonal view. I tried to replicate it like in this video:" https://www.youtube.com/watch?v=AOqeJtE04YM " but it doesn’t stay hardened in the programmed position.

Unbenannt
Here I select Transfrom to connect the Volume with the predefined Transform file.

Unbenannt1

Now the position of the volume in the red window is correct (and in the others too), I want to lock this transformation.

Unbenannt2

After hardening it and closing and opening the eye icon again, its shifted in at a new angel.

My main goal is to shift the voxels or slices according to an orthogonal view of my sample.

2

Can you expand the slice view toolbar and make sure that the orientation is not shown as “reformat”?

3

It is due to Slicer automatically setting the slice views to match the image axes by default. You can turn off this feature by right-clicking on the eye icon and unchecking “Reset… on show” checkboxes:

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