I’ve been working to do some tractography using slicerDMRI, but seems to encounter the same problem with all DWI files that i have as i failed to convert it to tensors. Here, I attached the .nrrd file of our DWI.
If you look at the header you can see that there’s only one volume (a baseline, with gradient 0,0,0). For tractography you typically need dozens of gradients at different angles.
Acquiring diffusion MRI in a form compatible with tractography is a complex process, and the detail vary a lot by scanner type. Generally speaking you would want dozens of gradient directions and usually there will be a standard sequence from the scanner vendor the provides good results.